Proof of Principle
Biosensia has demonstrated proof of principle for the RapiPlex platform components and concepts through successfully developing assays for:
- Drugs of Abuse using neat saliva
- C-Reactive Protein, Myoglobin and Troponin I using whole blood, plasma and serum
How RapiPlex works
RapiPlex’s assay test cartridge system has five distinct components:
- An assay strip
- A fluidic chip
- An outer cartridge
- A sample collection mechanism
- A fluorescence reader
The Assay Strip
This assay strip consists of a conjugate pad followed by a membrane pad.
The Fluidic Chip
This chip is designed to allow multiplex analysis from a single sample. It can contain up to 6 individual discrete tests on a single chip from a single test sample.
Each assay strip is placed and sealed into a discrete capillary channel. Thus in the context of a drugs of abuse assay chip, each channel represents an individual test, i.e. channel one is for Cocaine, channel two for Amphetamine, and so on.
The Cartridge
The fluidic chip containing the assay strips is assembled into the cartridge.
A window on one side of the cartridge allows the test and control zone portions of the strip to be measured using a fluorescence reader.
Sample Collection Mechanisms
Sample collection mechanisms have been devised for whole blood collection and saliva collection, filtration and dispensing into the assembled chip and cartridge.
Whole blood is diluted in buffer and then applied directly to the cartridge and chip assembly.
The saliva collection device is placed in the subject’s mouth. The device is then removed and inserted into the cartridge.
The hinged lid on the cartridge is used to extract the saliva from the collection device. The saliva runs into the channels to complete the assay.
Fluorescence Reader
The RapiPlex platform integrates a fluorescence reader for assay result read out.